Throughout the brain, neurons communicate with each other through synapses, where neuronal specializations—presynaptic terminals, equipped with neurotransmitter release machineries, and postsynapses, containing neurotransmitter receptors—are connected by a narrow synaptic cleft. The correct positioning of elements in these three compartments allows for the flow of information between neurons. A series of phenomena referred to as synaptic plasticity can modulate synaptic strength, which endows us with the ability to learn from and adapt to a changing environment. In our lab we combine biochemistry, molecular neurobiology and electrophysiology to understand the molecular mechanisms that govern synapse development, organization and plasticity. Our goal is to decipher how signaling elements of the widespread glutamatergic and endocannabinoid systems are organized at synapses, how this organization is dynamically regulated to enable synaptic plasticity and how its disruption can lead to pathological states.